More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chemokine(C-C motif) ligand 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human I-309(80-96aa WVQRHRKMLRHCPSKRK). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CCL1 |
Protein | C-C motif chemokine 1 |
Uniprot ID | P22362 |
Function | Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the intercrine beta (chemokine CC) family. |
Aliases | C-C motif chemokine 1 antibody|CCL1 antibody|CCL1_HUMAN antibody|Chemokine CC Motif Ligand 1 antibody|inflammatory cytokine I-309 antibody|P500 antibody|SCYA1 antibody|SISe antibody|small inducible cytokine A1 antibody|Small-inducible cytokine A1 antibody|T lymphocyte secreted protein I-309 antibody|T lymphocyte-secreted protein I-309 antibody|TCA3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-I-309 antibody, ASA-B0958, Western blotting
All lanes: Anti I-309 (ASA-B0958) at 0.5ug/ml
Lane 1: SCG Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 18KD
All lanes: Anti I-309 (ASA-B0958) at 0.5ug/ml
Lane 1: SCG Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 18KD
