More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hyaluronoglucosaminidase 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human HYAL2(142-157aa VRNWQDKDVYRRLSRQ), different from the related mouse and rat sequences by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HYAL2 |
Protein | Hyaluronidase-2(Hyal-2) |
Uniprot ID | Q12891 |
Function | Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. |
Tissue Specificity | Widely expressed. No expression detected in adult brain. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the glycosyl hydrolase 56 family. |
Aliases | Hyal 2 antibody|Hyal-2 antibody|Hyal2 antibody|HYAL2_HUMAN antibody|Hyaluronidase 2 antibody|Hyaluronidase 2 Precursor antibody|Hyaluronidase-2 antibody|Hyaluronoglucosaminidase 2 antibody|Hyaluronoglucosaminidase-2 antibody|LUCA 2 antibody|LUCA-2 antibody|LUCA2 antibody|Lung carcinoma protein 2 antibody|lysosomal hyaluronidase antibody|PH 20 homolog antibody|PH-20 homolog antibody|PH20 homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HYAL2 antibody, ASA-B0955, Western blotting
All lanes: Anti HYAL2 (ASA-B0955) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 54KD
Observed bind size: 54KD
All lanes: Anti HYAL2 (ASA-B0955) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 54KD
Observed bind size: 54KD
