More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| huntingtin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Huntingtin(3106-3121aa ELDRRAFQSVLEVVAA), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HTT |
Protein | Huntingtin |
Uniprot ID | P42858 |
Function | May play a role in microtubule-mediated transport or vesicle function. |
Tissue Specificity | Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. |
Sub-cellular localization | Cytoplasm. Nucleus. Note: The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington disease neurons. Shuttles between cytoplasm and nucleus in a Ran GTPase-independent manner. |
Sequence Similarities | Belongs to the huntingtin family. |
Aliases | AI256365 antibody|C430023I11Rik antibody|HD antibody|HD protein antibody|HDH antibody|HTT antibody|Huntingtin antibody|HUNTINGTON CHOREA antibody|Huntington disease protein antibody|IT 15 antibody|IT15 antibody|OTTMUSP00000026909 antibody|ZHD antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Huntingtin antibody, ASA-B0952, All Western blotting
All lanes: Anti-HTT(ASA-B0952) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Predicted bind size: 348KD
Observed bind size: 348KD
All lanes: Anti-HTT(ASA-B0952) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Predicted bind size: 348KD
Observed bind size: 348KD
