More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nerve growth factor(beta polypeptide) | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived human NGFB recombinant protein(Position: S122-A241). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NGF |
Protein | Beta-nerve growth factor |
Uniprot ID | P01138 |
Function | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the NGF-beta family. |
Aliases | Beta nerve growth factor antibody|Beta NGF antibody|Beta-nerve growth factor antibody|Beta-NGF antibody|HSAN5 antibody|MGC161426 antibody|MGC161428 antibody|Nerve growth factor beta antibody|Nerve growth factor beta polypeptide antibody|Nerve growth factor beta subunit antibody|NGF antibody|NGFB antibody|NID67 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-human NGF antibody, ASA-B0948, Western blotting
Lane 1: Recombinant Human NGFB Protein 10ng
Lane 2: Recombinant Human NGFB Protein 5ng
Lane 3: Recombinant Human NGFB Protein 2
Lane 1: Recombinant Human NGFB Protein 10ng
Lane 2: Recombinant Human NGFB Protein 5ng
Lane 3: Recombinant Human NGFB Protein 2
