More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| B-cell CLL/lymphoma 2 | Polyclonal | IgG | Rabbit | Human | ELISA, Neu, IP, WB | Immunogen affinity purified. |
Immunogen |
| E. coli-derived human BCL-2 recombinant protein(Position: M1-D211). |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BCL2 |
Protein | Apoptosis regulator Bcl-2 |
Uniprot ID | P10415 |
Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). |
Tissue Specificity | Expressed in a variety of tissues. |
Sub-cellular localization | Mitochondrion outer membrane. |
Sequence Similarities | Belongs to the Bcl-2 family. |
Aliases | Apoptosis regulator Bcl2 antibody|B cell CLL antibody|B cell lymphoma 2 antibody|B cell lymphoma protein 2 alpha isoform antibody|B cell lymphoma protein 2 antibody|B-cell CLL/lymphoma 2 antibody|B-cell leukemia/lymphoma 2 antibody|Bcl 2 antibody|bcl2a antibody|Leukemia /lymphoma, B-cell, 2 antibody|Oncogene B cell leukemia 2 antibody|Oncogene B-cell leukemia 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
| Neutralization | Human | Human | |
| Immunoprecipitation | Human | Human | |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
