More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock protein 90kDa alpha(cytosolic), class A member 1; heat shock protein 90kDa alpha(cytosolic), class B member 1 | Monoclonal | IgG | Mouse | Human, Mouse, Rabbit, Rat | WB | Ascites |
Immunogen |
| Heat shock protein 90(HSP90) from the water mold Achlya ambisexualis. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSP90AA1; HSP90AB1 |
Protein | Heat shock protein HSP 90-alpha; Heat shock protein HSP 90-beta |
Uniprot ID | Human(P07900), Mouse(P07901), Rat(P82995), Human(P08238), Mouse(P11499), Rat(P34058) |
Function | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Melanosome. Cell membrane. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Sequence Similarities | |
Aliases | HSP90A|HSPC1|HSPCA|HSP90B|HSPC2|HSPCB |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 2-4μg/ml | Human, mouse, rabbit, rat | AssaySolutio's ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
