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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| HNF1 homeobox A | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human HNF1 recombinant protein (Position: A431-Q631). Human HNF1 shares 93% and 92% amino acid (aa) sequences identity with mouse and rat HNF1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HNF1A |
Protein | Hepatocyte nuclear factor 1-alpha |
Uniprot ID | P20823 |
Function | Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'- GTTAATNATTAAC-3'. |
Tissue Specificity | Liver. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the HNF1 homeobox family. |
Aliases | Albumin proximal factor antibody|Hepatic nuclear factor 1 alpha antibody|Hepatic nuclear factor 1 antibody|Hepatic transcription factor 1 alpha antibody|Hepatic transcription factor 1 antibody|Hepatocyte nuclear factor 1-alpha antibody|HNF 1 antibody|HNF 1A antibody|HNF-1-alpha antibody|HNF-1A antibody|HNF1A antibody|HNF1A_HUMAN antibody|Interferon production regulator factor antibody|LF B1 antibody|LF B1 hepatic nuclear factor antibody|LFB 1 antibody|LFB1 antibody|LFB1 hepatic nuclear factor antibody|Liver specific transcription factor LF B1 antibody|Liver specific transcription factor LFB1 antibody|Liver-specific transcription factor LF-B1 antibody|Maturity onset diabetes of the young 3 antibody|MODY 3 antibody|MODY3 antibody|TCF 1 antibody|TCF-1 antibody|TCF1 antibody|Transcription factor 1 antibody|Transcription factor 1 hepatic antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HNF1 antibody, ASA-B0880, Western blotting
All lanes: Anti HNF1 (ASA-B0880) at 0.5ug/ml
WB: Recombinant Human HNF1 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti HNF1 (ASA-B0880) at 0.5ug/ml
WB: Recombinant Human HNF1 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- HNF1 antibody, ASA-B0880, Western blotting
All lanes: Anti HNF1 (ASA-B0880) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 67KD
Observed bind size: 67KD
All lanes: Anti HNF1 (ASA-B0880) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 67KD
Observed bind size: 67KD
