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HIF3 Antibody

ASA-B0867

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
hypoxia inducible factor 3, alpha subunit Polyclonal IgG Rabbit Human, Rat WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3(497-514aa DDDFQLNASEQLPRAYHR), different from the related rat and mouse sequences by five amino acids.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 HIF3A

Protein

 Hypoxia-inducible factor 3-alpha

Uniprot ID

 Q9Y2N7

Function

 Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression (PubMed:11573933, PubMed:16126907, PubMed:19694616, PubMed:20416395, PubMed:21069422). Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity).

Tissue Specificity

Expressed in vascular cells (at protein level) (PubMed:21069422). Expressed in kidney (PubMed:11573933, PubMed:19694616). Expressed in lung epithelial cells (PubMed:16775626). Expressed in endothelial cells (venous and arterial cells from umbilical cord and aortic endothelial cells) and in vascular smooth muscle cells (aorta) (PubMed:21069422). Strongly expressed in the heart, placenta, and skeletal muscle, whereas a weak expression profile was found in the lung, liver, and kidney (PubMed:12538644). Expressed weakly in cell renal cell carcinoma (CC-RCC) compared to normal renal cells (PubMed:16126907). Expression is down-regulated in numerous kidney tumor cells compared to non tumor kidney tissues (PubMed:16126907). Isoform 2 is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas and in numerous cancer cell lines (PubMed:20416395). Isoform 3 and isoform 4 are weakly expressed in heart, placenta, lung, liver, skeletal muscle and pancreas (PubMed:20416395). Isoform 4 is expressed in fetal tissues, such as heart, brain, thymus, lung, liver, skeletal kidney and spleen (PubMed:20416395). Isoform 3 is weakly expressed in fetal tissues, such as liver and kidney (PubMed:20416395).

Sub-cellular localization

 Nucleus.

Sequence Similarities

 Contains 1 bHLH (basic helix-loop-helix) domain.

Aliases

 Basic-helix-loop-helix-PAS protein MOP7 antibody|bHLHe17 antibody|Class E basic helix-loop-helix protein 17 antibody|HIF 3A antibody|HIF 3A4 antibody|HIF-3-alpha antibody|HIF3 alpha antibody|HIF3-alpha antibody|HIF3-alpha-1 antibody|HIF3A antibody|HIF3A_HUMAN antibody|Hypoxia Inducible Factor 3 alpha antibody|Hypoxia inducible factor 3 alpha subunit antibody|Hypoxia inducible factor three alpha antibody|Hypoxia-inducible factor 3-alpha antibody|Inhibitory PAS domain protein antibody|IPAS antibody|Member of PAS protein 7 antibody|MOP7 antibody|PAS domain-containing protein 7 antibody|PASD7 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Rat HumanAssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HIF3 antibody, ASA-B0867, Western blotting
WB: Rat Brain Tissue Lysate

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