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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| aryl hydrocarbon receptor nuclear translocator | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human HIF1 beta recombinant protein (Position: V416-E789). Human HIF1 beta shares 86% and 83% amino acid (aa) sequences identity with mouse and rat HIF1 beta, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | 19,271,911 |
Protein | Aryl hydrocarbon receptor nuclear translocator |
Uniprot ID | P27540 |
Function | Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 1 bHLH (basic helix-loop-helix) domain. |
Aliases | ARNT antibody|ARNT protein antibody|ARNT_HUMAN antibody|Aryl Hydrocarbon Receptor Nuclear Translocator antibody|bHLHe2 antibody|Class E basic helix-loop-helix protein 2 antibody|Dioxin Receptor antibody|Dioxin receptor nuclear translocator antibody|Drnt antibody|HIF 1 beta antibody|HIF 1beta antibody|HIF-1-beta antibody|HIF1-beta antibody|HIF1B antibody|HIF1beta antibody|Hypoxia Inducible Factor 1 antibody|Hypoxia inducible factor 1 beta antibody|Hypoxia-inducible factor 1-beta antibody|nuclear translocator antibody|Tango antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HIF1 beta antibody, ASA-B0866--1.jpg
All lanes: Anti HIF1 beta (ASA-B0866) at 0.5ug/ml
WB: Recombinant Human HIF1 beta Protein 0.5ng
Predicted bind size: 49KD
Observed bind size: 49KD
All lanes: Anti HIF1 beta (ASA-B0866) at 0.5ug/ml
WB: Recombinant Human HIF1 beta Protein 0.5ng
Predicted bind size: 49KD
Observed bind size: 49KD
Anti-HIF1 beta antibody, ASA-B0866--2.jpg
All lanes: Anti HIF1 beta (ASA-B0866) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: Jurkat Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane5: Colo320 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
All lanes: Anti HIF1 beta (ASA-B0866) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: Jurkat Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane5: Colo320 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
