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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hepatocyte growth factor (hepapoietin A; scatter factor) | Polyclonal | IgG | Rabbit | Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse HGF (33-64aa QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKK), different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HGF |
Protein | Hepatocyte growth factor |
Uniprot ID | Q08048 |
Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the peptidase S1 family. Plasminogen subfamily. |
Aliases | DFNB39 antibody|F TCF antibody|Fibroblast derived tumor cytotoxic factor antibody|Hepatocyte growth factor (hepapoietin A; scatter factor) antibody|Hepatocyte growth factor antibody|Hepatocyte growth factor beta chain antibody|Hepatocyte growth factor precursor antibody|Hepatopoeitin-A antibody|Hepatopoietin A antibody|Hgf antibody|HGF_HUMAN antibody|HGFB antibody|HPTA antibody|Lung fibroblast derived mitogen antibody|OTTHUMP00000161349 antibody|OTTHUMP00000206710 antibody|OTTHUMP00000206711 antibody|OTTHUMP00000206712 antibody|OTTHUMP00000206713 antibody|OTTHUMP00000206730 antibody|Scatter factor antibody|SF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HGF antibody, ASA-B0861, Western blotting
All lanes: Anti HGF (ASA-B0861) at 0.5ug/ml
WB: NIH Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
All lanes: Anti HGF (ASA-B0861) at 0.5ug/ml
WB: NIH Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
