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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| X-ray repair complementing defective repair in Chinese hamster cells 3 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human XRCC3(148-164aa HKRLQQLMAQQPRLRTD). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | XRCC3 |
Protein | DNA repair protein XRCC3 |
Uniprot ID | O43542 |
Function | Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts in the BRCA1-BRCA2- dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion. Note: Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair. |
Sequence Similarities | Belongs to the RecA family. RAD51 subfamily. |
Aliases | CMM6 antibody|DNA repair protein XRCC3 antibody|RAD51 like antibody|X ray repair complementing defective repair in Chinese hamster antibody|X ray repair complementing defective repair in Chinese hamster cells 3 antibody|X ray repair cross complementing protein 3 antibody|X-ray repair cross-complementing protein 3 antibody|XRCC 3 antibody|XRCC3 antibody|XRCC3_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-XRCC3 antibody, ASA-B2000, Western blotting
All lanes: Anti XRCC3 (ASA-B2000) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti XRCC3 (ASA-B2000) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
