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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| wingless-type MMTV integration site family, member 3 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Wnt3 (185-207aa AMNKHNNEAGRTTILDHMHLKCK), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | WNT3 |
Protein | Proto-oncogene Wnt-3 |
Uniprot ID | P56703 |
Function | Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | |
Aliases | Int 4 antibody|INT4 antibody|MGC131950 antibody|MGC138321 antibody|MGC138323 antibody|Proto-oncogene Int-4 homolog antibody|Proto-oncogene Wnt-3 antibody|wingless type MMTV integration site family member 3 antibody|Wnt 3 proto oncogene protein antibody|WNT 3 proto oncogene protein precursor antibody|wnt3 antibody|WNT3_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- WNT3 antibody, ASA-B1991, Western blotting
All lanes: Anti WNT3 (ASA-B1991) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti WNT3 (ASA-B1991) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
