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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| wingless-type MMTV integration site family member 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt2 (56-81aa HRHPDVMRAISQGVAEWTAECQHQFR), different from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | WNT2 |
Protein | Protein Wnt-2 |
Uniprot ID | P09544 |
Function | Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. |
Tissue Specificity | Expressed in brain in the thalamus, in fetal and adult lung and in placenta. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | |
Aliases | Int 1 related protein antibody|Int 1 related protein antibody|Int-1-like protein 1 antibody|Int-1-related protein antibody|INT1L 1 antibody|INT1L1 antibody|IRP antibody|IRP protein antibody|IRP protein antibody|ONCOGENE INT1 LIKE 1 antibody|Protein Wnt-2 antibody| Secreted growth factor antibody|Wingless type MMTV integration site family member 2 antibody|WNT 2 antibody|WNT 2 antibody| WNT2 antibody|WNT2_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-WNT2 antibody, ASA-B1988, Western blotting
All lanes: Anti WNT2 (ASA-B1988) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti WNT2 (ASA-B1988) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
