More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| vitamin D(1,25- dihydroxyvitamin D3) receptor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Vitamin D Receptor(389-404aa DLRSLNEEHSKQYRCL), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | VDR |
Protein | Vitamin D3 receptor |
Uniprot ID | P11473 |
Function | Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR1 subfamily. |
Aliases | 1 25 dihydroxyvitamin D3 receptor antibody|1 antibody|1,25-@dihydroxyvitamin D3 receptor antibody|125 dihydroxyvitamin D3 receptor antibody|25-dihydroxyvitamin D3 receptor antibody|Member 1 antibody|NR1I1 antibody|Nuclear receptor subfamily 1 group I member 1 antibody|VDR antibody|VDR_HUMAN antibody|Vitamin D(1,25- dihydroxyvitamin D3) receptor antibody|Vitamin D hormone receptor antibody|Vitamin D nuclear receptor variant 1 antibody|Vitamin D receptor antibody|Vitamin D3 receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Vitamin D Receptor antibody, ASA-B1979, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
