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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| fms-related tyrosine kinase 1(vascular endothelial growth factor/vascular permeability factor | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human VEGF Receptor 1(1299-1317aa HVSEGKRRFTYDHAELERK). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FLT1 |
Protein | Vascular endothelial growth factor receptor 1(VEGFR-1) |
Uniprot ID | P17948 |
Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 7 has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion. |
Tissue Specificity | Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC). |
Sub-cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Endosome. Note: Autophosphorylation promotes ubiquitination and endocytosis. |
Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. |
Aliases | EC 2.7.10.1 antibody|Flt 1 antibody|FLT antibody|Flt-1 antibody|Flt1 antibody|Fms like tyrosine kinase 1 antibody|Fms related tyrosine kinase 1(vascular endothelial growth factor/vascular permeability factor receptor) antibody|Fms related tyrosine kinase 1 antibody|Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody|Fms-like tyrosine kinase 1 antibody|FRT antibody|Soluble VEGF receptor 1 14 antibody|Soluble VEGFR1 variant 2 antibody|Soluble VEGFR1 variant 21 antibody|Tyrosine protein kinase FRT antibody|Tyrosine protein kinase receptor FLT antibody|Tyrosine-protein kinase FRT antibody|Tyrosine-protein kinase receptor FLT antibody|Vascular endothelial growth factor receptor 1 antibody|Vascular endothelial growth factor receptor 1 Precursor antibody|Vascular endothelial growth factor vascular permeability factor receptor antibody|Vascular permeability factor receptor 1 antibody|Vascular permeability factor receptor antibody|VEGFR 1 antibody|VEGFR-1 antibody|VEGFR1 antibody|VGFR1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-VEGF Receptor 1 antibody, ASA-B1964, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: SGC Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: SGC Cell Lysate
Lane 3: MM231 Cell Lysate
