More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ubiquitin-conjugating enzyme E2I | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human UBE2I(108-122aa IKQILLGIQELLNE), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | UBE2I |
Protein | SUMO-conjugating enzyme UBC9 |
Uniprot ID | P63279 |
Function | Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. |
Tissue Specificity | Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. |
Sub-cellular localization | Nucleus. Cytoplasm. Note: Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity). |
Sequence Similarities | Belongs to the ubiquitin-conjugating enzyme family. |
Aliases | C358B7.1 antibody|Homologous to yeast UBC9 antibody|p18 antibody|SUMO 1 conjugating enzyme antibody|SUMO 1 protein ligase antibody|SUMO conjugating enzyme UBC9 antibody|SUMO protein ligase antibody|SUMO-conjugating enzyme UBC9 antibody|SUMO-protein ligase antibody|SUMO1 conjugating enzyme antibody|SUMO1 protein ligase antibody|UBC 9 antibody|UBC9 antibody|UBC9 homolog antibody|UBC9_HUMAN antibody|UBCE 9 antibody|UBCE9 antibody|UBE 2I antibody|Ube2i antibody|Ubiquitin carrier protein 9 antibody|Ubiquitin carrier protein antibody|Ubiquitin carrier protein I antibody|Ubiquitin conjugating enzyme 9 antibody|Ubiquitin conjugating enzyme E2I(homologous to yeast UBC9) antibody|Ubiquitin conjugating enzyme E2I(UBC9 homolog, yeast) antibody|Ubiquitin conjugating enzyme E2I antibody|Ubiquitin conjugating enzyme UbcE2A antibody|Ubiquitin like protein SUMO 1 conjugating enzyme antibody|Ubiquitin like protein SUMO1 conjugating enzyme antibody|Ubiquitin protein ligase E2I antibody|Ubiquitin protein ligase I antibody|Ubiquitin-conjugating enzyme E2 I antibody|Ubiquitin-protein ligase I antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-UBE2I/UBC9 antibody, ASA-B1946, All Western blotting
All lanes: Anti-UBE2I(ASA-B1946) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 40ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 3: Rat Pancreas Tissue Lysate at 40ug
Predicted bind size: 18KD
Observed bind size: 18KD
All lanes: Anti-UBE2I(ASA-B1946) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 40ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 3: Rat Pancreas Tissue Lysate at 40ug
Predicted bind size: 18KD
Observed bind size: 18KD
