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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tuberous sclerosis 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Tuberin(1605-1620aa QFTYCWHDDIMQAVFH), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TSC2 |
Protein | Tuberin |
Uniprot ID | P49815 |
Function | In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase- activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. |
Tissue Specificity | Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta. |
Sub-cellular localization | Cytoplasm. Membrane; Peripheral membrane protein. Note: At steady state found in association with membranes. |
Sequence Similarities | Contains 1 Rap-GAP domain. |
Aliases | FLJ43106 antibody|LAM antibody|OTTHUMP00000158940 antibody|OTTHUMP00000198394 antibody|OTTHUMP00000198395 antibody|TSC2 antibody|TSC2_HUMAN antibody|TSC4 antibody|Tuberin antibody|Tuberous sclerosis 2 antibody|Tuberous sclerosis 2 protein antibody|Tuberous sclerosis 2 protein homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Tuberin antibody, ASA-B1942, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: HEPA Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: HEPA Cell Lysate
