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TRPV6 Antibody

ASA-B1938

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
transient receptor potential cation channel, subfamily V, member 6 Polyclonal IgG Rabbit Human, Mouse, Rat WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TRPV6(18-37aa KFCRWFQRRESWAQSRDEQN), different from the related rat sequence by one amino acid, and from the mouse sequence by two amino acids.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 TRPV6

Protein

 Transient receptor potential cation channel subfamily V member 6

Uniprot ID

 Q9H1D0

Function

 Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin- dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).

Tissue Specificity

Expressed at high levels in the gastrointestinal tract, including esophagus, stomach, duodenum, jejunum, ileum and colon, and in pancreas, placenta, prostate and salivary gland. Expressed at moderate levels in liver, kidney and testis. Expressed in trophoblasts of placenta villus trees (at protein level)(PubMed:23612980). Expressed in locally advanced prostate cancer, metastatic and androgen-insensitive prostatic lesions but not detected in healthy prostate tissue and benign prostatic hyperplasia.

Sub-cellular localization

 Cell membrane.

Sequence Similarities

 Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV6 sub-subfamily.

Aliases

 ABP/ZF antibody|ABPZF antibody|Alu binding protein with zinc finger domain antibody|Calcium channel CaT1 antibody|Calcium transport protein 1 antibody|CaT L antibody|CaT Like antibody|CaT-L antibody|CaT-like antibody|CaT1 antibody|ecac antibody|ECaC2 antibody|Epithelial apical membrane calcium transporter/channel CaT1 antibody|Epithelial calcium channel 2 antibody|Epithelial calcium channel antibody|HSA277909 antibody|LP6728 antibody|MGC162545 antibody|NUDC antibody|Transient receptor potential cation channel subfamily V member 6 antibody|TrpV6 antibody|TRPV6_HUMAN antibody|ZFAB antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRPV6 antibody, ASA-B1938, Western blotting
All lanes: Anti TRPV6 (ASA-B1938) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD

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