More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CREB regulated transcription coactivator 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TORC2(595-611aa QDPHTFNHQNLTHCSRH), different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CRTC2 |
Protein | CREB-regulated transcription coactivator 2 |
Uniprot ID | Q53ET0 |
Function | Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). |
Tissue Specificity | Most abundantly expressed in the thymus. Present in both B and T-lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas. |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus. |
Sequence Similarities | Belongs to the TORC family. |
Aliases | CREB regulated transcription coactivator 2 antibody|CREB-regulated transcription coactivator 2 antibody|CRTC2 antibody|CRTC2_HUMAN antibody|RP11-422P24.6 antibody|TORC-2 antibody|torc2 antibody|Transducer of CREB protein 2 antibody|Transducer of regulated cAMP response element-binding protein antibody|Transducer of regulated cAMP response element-binding protein(CREB) 2 antibody|Transducer of regulated cAMP response element-binding protein 2 antibody|Transducer of regulated CREB protein 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TORC2 antibody, ASA-B1898, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human CRTC2, 29.3KD(162aa tag+ L585-Q693)
Lane 1: Recombinant Human CRTC2 Protein 10ng
Lane 2: Recombinant Human CRTC2 Protein 5ng
Lane 3: Recombinant Human CRTC2 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human CRTC2, 29.3KD(162aa tag+ L585-Q693)
Lane 1: Recombinant Human CRTC2 Protein 10ng
Lane 2: Recombinant Human CRTC2 Protein 5ng
Lane 3: Recombinant Human CRTC2 Protein 2.5ng
