| Formulation |
SNRPD is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
| Purity |
Greater than 80% as determined by SDS-PAGE. |
| Applications |
Western blot with:a) Anti-Sm positive patient sera.b) Monoclonal anti-hexa-His-tag antibody. |
| Description |
SNRPD Human Recombinant produced in SF9 insect cells, is a mixed protein complex containing the SNRPD1 (14,306 Dalton), SNRPD2 (14,773 Dalton) and SNRPD3 (14,739 Dalton) proteins. Each protein (SNRPD1+ SNRPD2+ SNRPD3) is individually fused to a -6xHis tag at N-terminus and purified by proprietary chromatographic techniques. |
| Expression host |
Sf9 insect cells. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions |
1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels). |
| Coating concentration |
0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups. |
View larger
