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Small Nuclear Ribonucleoprotein Polypeptide D3 Human Recombinant, Sf9 ( SNRPD3 Human, Sf9 )
Data sheet
| Formulation | SNRPD3 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
| Purity | Greater than 80% as determined by SDS-PAGE. |
| Applications | Western blot with anti-Sm positive patient sera. |
| Description | SNRPD3 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 14,739 Dalton. SNRPD3 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques. |
| Protein Background | SNRPD3 is a small nuclear ribonucleoprotein (snRNPs) that contains the spliceosome in eukaryotes. SNRPD3 is essential for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing happens in this locus and two transcript variants encoding the same protein were branded. |
| Expression host | Sf9 insect cells. |
| Synonyms | Small nuclear ribonucleoprotein D3 polypeptide 18kDa, Sm-D3, snRNP core protein D3. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera). |
| Coating concentration | 0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |
More info
Sm-D3, snRNP core protein D3, Small nuclear ribonucleoprotein D3 polypeptide 18kDa