Data sheet
| Formulation | The LSM3 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, 2mM DTT and 20% glycerol. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Description | LSM3 Human Recombinant produced in E. coli is a single polypeptide chain containing 125 amino acids (1-102) and having a molecular mass of 14.3kDa.LSM3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background | LSM3 is a member of snRNP Sm proteins family which were identified in a large number of organisms based on sequence homology. Sm-like proteins have a Sm sequence motif that holds 2 regions detached by a linker of variable length that fold as a loop. The Sm-like proteins form a stable heteromer found in tri-snRNP particles that are vital for pre-mRNA splicing. |
| Expression host | E.coli. |
| Synonyms | LSM3 homolog U6 small nuclear RNA associated (S. cerevisiae), U6 snRNA-associated Sm-like protein LSm3, SMX4, USS2, YLR438C. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MGSMADDVDQ QQTTNTVEEP LDLIRLSLDE RIYVKMRNDR ELRGRLHAYD QHLNMILGDV EETVTTIEID EETYEEIYKS TKRNIPMLFV RGDGVVLVAP PLRVG |
More info
SMX4, U6 snRNA-associated Sm-like protein LSm3, USS2, YLR438C, LSM3 homolog U6 small nuclear RNA associated (S. cerevisiae)