Data sheet
| Formulation | The LAT solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 1mM DTT. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Description | LAT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (28-233) and having a molecular mass of 24.4 kDa (Molecular weight on SDS-PAGE will appear higher).LAT is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background | LAT is a vital membrane protein that is phosphorylated by ZAP-70/Syk protein tyrosine kinases succeeding activation of the T-cell antigen receptor (TCR) signal transduction pathway. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. |
| Expression host | E.coli. |
| Synonyms | Linker for activation of T cells, LAT1, 36 kDa phospho-tyrosine adapter protein, p36-38, pp36, linker for activation of T cells transmembrane adaptor, linker for activation of T-cells family member 1. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MHCHRLPGSY DSTSSDSLYP RGIQFKRPHT VAPWPPAYPP VTSYPPLSQP DLLPIPRSPQ PLGGSHRTPS SRRDSDGANS VASYENEEPA CEDADEDEDD YHNPGYLVVL PDSTPATSTA APSAPALSTP GIRDSAFSME SIDDYVNVPE SGESAEASLD GSREYVNVSQ ELHPGAAKTE PAALSSQEAE EVEEEGAPDY ENLQELN |
More info
36 kDa phospho-tyrosine adapter protein, LAT1, linker for activation of T cells transmembrane adaptor, linker for activation of T-cells family member 1, p36-38, pp36, Linker for activation of T cells