Complement Component C5a Mouse Recombinant ( C5a Mouse )

Data sheet

Formulation

Mouse C5a was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.5 and 350mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

C5a Mouse Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa.The Mouse C5a is purified by proprietary chromatographic techniques.

Protein Background

Mouse Complement 5a (C5a) is a glycoprotein which is a member of a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 77 a.a. peptide created by the C5a convertase proteolytic cleavage of C5 ? chain in the classical

Expression host

Escherichia Coli.

Synonyms

Complement C5, Hemolytic complement, C5, Hc, He, C5a.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse C5a should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Leu-His-Leu-Leu.

Biological Activity

The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml.