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Calbindin-2 Human Recombinant ( CALB2 Human )
DescriptionCALB2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 211 amino acids (1-271 a.a.) and having a molecular mass of 33.7kDa.The CALB2 is fused to a 20 amino acid His-Tag at N-terminus and
Data sheet
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Formulation | The CALB2 1mg/ml protein solution contains 20mM Tris-HCl buffer pH-8 & 10% glycerol. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Description | CALB2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 211 amino acids (1-271 a.a.) and having a molecular mass of 33.7kDa.The CALB2 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. |
| Protein Background | Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas. |
| Expression host | Escherichia Coli. |
| Synonyms | Calretinin, CR, CALB2, CAB29, CAL2, CaBP29K, 29 kDa calbindin. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MAGPQQQPPY LHLAELTASQ FLEIWKHFDA DGNGYIEGKE LENFFQELEK ARKGSGMMSK SDNFGEKMKE FMQKYDKNSD GKIEMAELAQ ILPTEENFLL CFRQHVGSST EFMEAWRKYD TDRSGYIEAN ELKGFLSDLL KKANRPYDEP KLQEYTQTIL RMFDLNGDGK LGLSEMSRLL PVQENFLLKF QGMKLTSEEF NAIFTFYDKD RSGYIDEHEL DALLKDLYEK NKKEMNIQQL TNYRKSVMSL AEAGKLYRKD LEIVLCSEPP M. |
| Antibody Properties | Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Available Conjugates | Blocking and staining antibody. For staining, use 10ul/1,000,000 cells. Titer for blocking LPS binding should be determined by the investigator. |
| Immunological function | This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10ul/106 cells. |