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Centromere Protein A Human Recombinant ( CENPA Human )
DescriptionCENPA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENPA is expressed with a -6x His tag and purified by proprietary chromatographic techniques.SourceSf9 insect
Data sheet
| Formulation | CENPA is supplied in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Applications | Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera. |
| Description | CENPA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENPA is expressed with a -6x His tag and purified by proprietary chromatographic techniques. |
| Protein Background | Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.CENPA is another important centromeric autoantigen in addition to CENPB: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENPA antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENPB-autoantibody negative patients. |
| Expression host | Sf9 insect cells. |
| Synonyms | Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA. |
| Reagent Appearance | Sterile Filtered liquid formulation. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera). |
| Coating concentration | 0.15-0.4 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination. |