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Der F1 Mosaic Protein Recombinant ( Der F1 )
DescriptionThe E.Coli derived recombinant protein contains the Der F1 epitopes 25-51, 74-95, 189-209 amino acids.MethodPurified by proprietary chromatographic technique.PurityProtein is >95% pure as determined by 10% PAGE (coomassie staining) and
Data sheet
| Storage | Der F1 although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Formulation | 60mM NaCl, 50mM Tris-HCl pH 8.0 and 1.2M Urea. |
| Purity | Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC. |
| Description | The E.Coli derived recombinant protein contains the Dermatophagoides farina Der F1 full length protein (a.a. 1-320) and fused to a 6 His Tag at C-terminus, having a total Mw of 36kDa, pI 5.88. |
| Protein Background | DERF1 is a thiol protease that hydrolyzes proteins, with a preference for Phe or basic residues. DERF1 is a C1 peptidase family member. DERF1 has extensive endopeptidase specificity. DERF1 causes an allergic reaction in humans. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis. |
| Expression host | E.Coli. |
| Reagent Appearance | Sterile filtered colorless solution. |