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DnaJ ( HSP40 ) E.Coli Recombinant ( DnaJ E.Coli )
DescriptionRecombinant Dna-J produced in E.Coli is a single, non-glycosylated polypeptide chain containing 376 amino acids and having a molecular mass of 41.1 kDa.SourceEscherichia Coli.Physical AppearanceSterile filtered colorless
Data sheet
| Formulation | The DnaJ contains 25mM Tris-HCl buffer (pH 7.5), 100mM NaCl, 5mM DTT and 10% Glycerol. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Description | Recombinant Dna-J produced in E.Coli is a single, non-glycosylated polypeptide chain containing 376 amino acids and having a molecular mass of 41.1 kDa. |
| Protein Background | DnaJ, Heat shock protein, functions in association with DnaK(Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues. |
| Expression host | Escherichia Coli. |
| Synonyms | HSP-40, HSP40, DnaJ, DNAJB1, HSPF1, Hdj1, Chaperone protein dnaJ, Heat shock protein J, groP, b0015, JW0014. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |