Coagulation Factor VIIa Human Recombinant ( F7 Human )

Data sheet

Formulation

The protein 1 mg/ml was lyophilized after from a sterile solution containing 10mg sucrose pH-6.

Solubility

It is recommended to reconstitute the lyophilized Factor-VIIa in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by analysis by SDS-PAGE.

Description

Factor VIIa Human Recombinant produced in BHK is a glycosylated polypeptide two-chain dimer consisting of 406 amino acids with a molecular weight of 50kD.The Factor-VIIa is purified by proprietary chromatographic techniques.

Protein Background

Coagulation factor VII is a vitamin K-dependent factor which is essential for hemostasis. It circulates in the blood as a zymogen which is later converted to an active form by factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon acti

Expression host

BHK cells (Baby Hamster Kidney Cells).

Synonyms

Coagulation factor VII, EC 3.4.21.21, Serum prothrombin conversion accelerator, SPCA, Proconvertin, Eptacog alfa, F7.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Factor-VIIa although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Factor-VIIa should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Biological Activity

The potency per mg was tested and found to be 50,000Units/mg.