GLI Pathogenesis-Related 2 Human Recimbinant ( GLIPR2 Human )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The GLIPR2 1mg/ml protein contains 20mM Tris-HCl pH-8, 1mM DTT, 0.1M DTT and 10% Glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

GLIPR2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids (1-154) & having a molecular mass of 19.3 kDa.The GLIPR2 is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chro

Protein Background

GLIPR2 is linked to plant pathogenesis-related (PR-1) proteins, which are upregulated in reaction to pathogen attack. GLIPR2 is localized within lipid-enriched microdomains on the cytosolic side of the endomembrane system. GLIPR2 is closely attached to me

Expression host

Escherichia Coli.

Synonyms

GAPR-1, Golgi-associated plant pathogenesis-related protein 1, GAPR1, GLIPR-2, Golgi-associated PR-1 protein.

Stability

Store GLIPR2 at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGKSASKQFH NEVLKAHNEY RQKHGVPPLK LCKNLNREAQ QYSEALASTR ILKHSPESSR GQCGENLAWA SYDQTGKEVA DRWYSEIKNY NFQQPGFTSG TGHFTAMVWK NTKKMGVGKA SASDGSSFVV ARYFPAGNVV NEGFFEENVL PPKK.

Physical Appearence

Sterile filtered colorless solution.

Antibody Properties

Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1 : 20,000 dilution will yield >1 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).