View larger
Heat-Responsive Protein 12 Human Recombinant ( HRSP12 Human )
DescriptionHRSP12 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids (1-137 a.a.) and having a molecular mass of 16.6kDa. The HRSP12 is
Data sheet
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Formulation | The HRSP12 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Description | HRSP12 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids (1-137 a.a.) and having a molecular mass of 16.6kDa. The HRSP12 is purified by proprietary chromatographic techniques. |
| Protein Background | HRSP12 (UK114) is an endoribonuclease found mainly in the human adult kidney and liver, and which is responsible for inhibiting translation by cleaving mRNA. HRSP12 cleaves phosphodiester bonds only in single-stranded RNA. HRSP12 may be an important biomarker for heptatic carcinoma. |
| Expression host | Escherichia Coli. |
| Synonyms | Ribonuclease UK114, 14.5 kDa translational inhibitor protein, p14.5, Heat-responsive protein 12, UK114 antigen homolog, HRSP12, PSP. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | HRSP12 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL. |
| Antibody Properties | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
| Introductionn | 1:1,000 dilution will block T cell proliferation induced by specific Ag or concanavaline A. 10ul will stain 106 IL-R positive cells for FACS analysis. |