Heat Shock Transcription Factor-1 Human Recombinant ( HSF1 Human )

Data sheet

Formulation

The HSF1 protein (1mg/ml) contains 20mM Tris pH-8, 50mM NaCl and 1mM DTT.

Purity

Greater than 75.0% as determined by SDS-PAGE.

Description

Recombinant Human HSF1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing a total of 549 amino acids (1-529) and having a molecular mass of 59.4kDa.The HSF1 protein is fused to a 20 aa His-Tag at N-terminus.

Protein Background

The transcription of heat-shock genes is rapidly induced after temperature stress in response to environmental changes, and it is also involved in oogenesis, spermatogenesis, and placental development. Hsp90, is a major repressor of HSF1 gene. HSF1 is kno

Expression host

Escherichia Coli.

Synonyms

HSF-1, HSF1, HSTF-1, HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MDLPVGPGAA GPSNVPAFLT KLWTLVSDPD TDALICWSPS GNSFHVFDQG QFAKEVLPKY FKHNNMASFV RQLNMYGFRK VVHIEQGGLV KPERDDTEFQ HPCFLRGQEQ LLENIKRKVT SVSTLKSEDI KIRQDSVTKL LTDVQLMKGK QECMDSKLLA MKHENEALWR EVASLRQKHA QQQKVVNKLI QFLISLVQSN RILGVKRKIP LM