View larger
Heat Shock Protein 104 Saccharomyces cerevisiae Recombinant ( HSP104 Saccharomyces )
DescriptionRecombinant HSP104 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 908 amino acids and having a molecular mass of 102 kDa.SourceSaccharomyces cerevisiae.Physical AppearanceSterile filtered colorless
Data sheet
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Formulation | The HSP-104 protein solution contains 20mM Tris-HCl, pH 7.4,100mM NaCl, 2mM EDTA and 5% Glycerol. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Description | Recombinant HSP104 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 908 amino acids and having a molecular mass of 102 kDa. |
| Protein Background | HSP104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 can protect yeast cells against high temperature and high concentration of ethanol but mutation studies have shown this protein is not required for normal growth. Hsp104 was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. |
| Expression host | Saccharomyces cerevisiae. |
| Synonyms | Heat shock protein 104, Protein aggregation-remodeling factor HSP104, HSP104, YLL026W, L0948. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Antibody Properties | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
| Protein content | By direct ELISA, 1:5,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |