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HUS1 Checkpoint Homolog Human Recombinant ( HUS1 Human )
DescriptionHUS1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 300 amino acids (1-280a.a.) and having a molecular mass of 33.8kDa.HUS1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary
Data sheet
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Formulation | The HUS1 protein solution (0.25mg/1ml) is formulated in . In 20 mM Tris-HCl Buffer (pH 8.0), 100 mM NaCl and 40% Glycerol. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Description | HUS1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 300 amino acids (1-280a.a.) and having a molecular mass of 33.8kDa.HUS1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background | HUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. HUS1 protein connects with Rad9 and Rad1 to form the 9-1-1(RAD9-RAD1-HUS1) complex, that confines to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response. |
| Expression host | Escherichia Coli. |
| Synonyms | HUS1 Checkpoint Homolog (S. pombe), Checkpoint Protein HUS1, Hus1+-like protein. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS |