Listeriolysin-O PEST free Recombinant ( LLO PEST free )

Catalog # PT_41425

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$327.00
DescriptionRecombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence. LLO-PEST minus is composed of 471 amino acids, starting from amino acid 60 to amino acid 529, with the addition of a methionine in its
Product Size: 10ug
Quantity

Data sheet

Formulation
The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Applications
a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6).
Description
Recombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence. LLO-PEST minus is composed of 471 amino acids, starting from amino acid 60 to amino acid 529, with the addition of a methionine in its NH2-terminus.
Protein Background
Listeriolysin O (aka LLO) is a hemolysin produced by Listeria monocytogenes bacteria, the pathogen responsible for causing listeriosis. The toxin may be regarded as a virulence factor, since it is crucial for the virulence of L. monocytogenes. LLO is a si
Expression host
Escherichia Coli.
Synonyms
Listeriolysin-O, LLO, hlyA.
Reagent Appearance
Sterile Filtered clear solution.
Stability
LLO although stable at 4°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Activity
7x104 HU/mg. 2mM DTT could be use to reactivate the toxin.
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