Myoglobin His Human Recombinant ( Myoglobin His Human )

Data sheet

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.

Formulation

The MB protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)1mM DTT, 100mM NaCl and 20% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

MB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids (1-154a.a.) and having a molecular mass of 19.3kDa.MB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Protein Background

Myoglobin, mitochondrial precursor is a cytosolic oxygen-binding protein in charge of the storage and diffusion of oxygen within myocytes. The largest expression of MB is in the skeletal and cardiac muscle. MB operates in various functions in muscular ox

Expression host

Escherichia Coli.

Synonyms

PVALB, MGC13548.

Reagent Appearance

Sterile Filtered clear solution.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGLSDGEWQL VLNVWGKVEA DIPGHGQEVL IRLFKGHPET LEKFDKFKHL KSEDEMKASE DLKKHGATVL TALGGILKKK GHHEAEIKPL AQSHATKHKI PVKYLEFISE CIIQVLQSKH PGDFGADAQG AMNKALELFR KDMASNYKEL GFQG