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Proliferating Cell Antigen Human Recombinant, Sf9 ( PCNA Human, Sf9 )
DescriptionPCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 29,794 Dalton. PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.SourceSf9 insect
Data sheet
| Formulation | Recombinant Human PCNA is supplied in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Applications | Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera. |
| Description | PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35 kDa. PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. |
| Protein Background | Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis.Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity ( |
| Expression host | Sf9 insect cells. |
| Synonyms | Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera. |
| Coating concentration | 0.5-1.1 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination. |