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Soybean P34 Protein Recombinant ( Soybean P34 )
DescriptionThe E.Coli derived recombinant protein contains Soybean P34 Protein epitopes 214-261, 351-379 amino acids. The protein is fused to a GST tag at N-terminus.MethodPurified by proprietary chromatographic technique.PurityProtein is >95% pure
Data sheet
| Storage | Soybean P34 although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Formulation | 50mM Tris-HCl, pH 8.0 and 60mM NaCl. |
| Purity | Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC. |
| Description | The E.Coli derived recombinant protein contains Soybean P34 Protein full length, having an Mw of 30kDa. The protein is fused to a His tag at C-terminus. |
| Protein Background | The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 23% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a ~32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons. |
| Expression host | E.Coli. |
| Reagent Appearance | Sterile filtered colorless solution. |