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SP100 Recombinant Human ( SP100 Human )
DescriptionRecombinant Sp100 antigen is a full-length cDNA coding having a molecular mass of 54,662 Dalton (pH 5.0). Sp100 protein is fused to a hexa-histidine purification tag.SourceSf9 insect cells.FormulationSp100 is supplied in 16mM
Data sheet
| Formulation | Sp100 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol. |
| Purity | Greater than 80% as determined by SDS-PAGE. |
| Applications | Western blot with PBC sera or monoclonal anti-hexa-His-tag antibody. |
| Description | Recombinant Sp100 antigen is a full-length cDNA coding having a molecular mass of 54,662 Dalton (pH 5.0). Sp100 protein is fused to a hexa-histidine purification tag. |
| Protein Background | SP100 protein is Homodimer which takes part in the control of gene expression. SP100 interacts with members of the HP1 family of nonhistone chromosomal protein, such as CBX5 and CBX3 via the PxVxL motif and also interacts with Epstein-Barr virus EBNA-LP.The major isoform Sp100-A, has a calculated MW of 54 kDa, but exhibits aberrant electrophoretic mobilities, with an apparent MW OF 100 kDa. |
| Expression host | Sf9 insect cells. |
| Synonyms | Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, Speckled 100 kDa, SP100-HMG nuclear autoantigen, FLJ00340, Lysp100b. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Coating concentration | 0.5-0.9 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |