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Thioredoxin Yeast Recombinant ( TXN1 Yeast )
DescriptionThioredoxin Yeast Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kDa.SourceEscherichia Coli.Physical AppearanceSterile Lyophilized Powder.FormulationEach mg of protein
Data sheet
| Formulation | Each mg of protein contains 20mM phosphate buffer pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized TRX in sterile 18M-cm H2O. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Description | Thioredoxin Yeast Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kDa. |
| Protein Background | Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. |
| Expression host | Escherichia Coli. |
| Synonyms | Thioredoxin-1, Thioredoxin I, TR-I, Thioredoxin-2, TRX1, TRX2, YLR043C. |
| Reagent Appearance | Sterile Lyophilized Powder. |
| Stability | TRX although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze thaw cycles. |
| Biological Activity | TRX activity is assayed by measuring the change in absorbance at 650 nm at 25°C using 0.13uM bovine insulin containing 0.33mM DTT (pH 6.5).The specific activity was found to be 5.8units/mg. |