Data sheet
| Size | 2.5 ml |
| Storage | Keep frozen at -70℃. SeaGrow is subject to oxidation and enzyme activity at temperatures above -70℃. Thaw SeaGrow in a 3-6℃ water bath or under refrigeration. Thaw only the amount needed or store in use-size aliquots to avoid repeated freezing and thawing. |
| Product Type | Fish Blocking Reagent |
| Product Category | Blocking Buffer |
| Format | Solution |
| Catalog Number | ND-R0552 |
| Preservative | None |
| Applications | Zebrafish Cell Culture |
| Comments | SeaGrow is derived from aseptically drawn whole blood and plasma products from salmonids. SeaGrow does not contain mammalian proteins. Although the immune system of fish is similar to other vertebrates, fish antibodies are unlike those of mammals and do not cross-react. ENDOTOXIN: |
| Description | Fish serum product used for the in vitro growth of cells |
| Dilution & Recommended Usage | SeaGrow has not been heat-treated, and will gel if heated alone. To prevent gelling, add a minimum of an equal volume of defined media to SeaGrow before heating to 55C for 30 minutes. Depending on your specific application, addition of at least twice the volume of defined media may be necessary to prevent gelling. Alternatively, users may dilute SeaGrow to the final working concentration prior to heating. FOR FISH CELLS AND INSECT CELLS: 1. Try SeaGrow at the 2.5% or 5% level if you have been using 10% or more FBS. 2. Heat-treatment may not be necessary for all cell lines.3. Sterile filtration to 0.2 micron after combination with defined media is recommended prior to use in culture. |
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