Rat EGF ELISA Kit

Kit Components

 1. Recombinant Rat EGF standard: 2 vials

 2. One 96-well plate coated with Rat EGF  Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1

10. Washing solution (20x): 25 mL x1

Background

Epidermal growth factor (EGF) is the founding member of the EGF family which structurally features the EGF-like domain containing three intramolecular disulfide bonds formed by six similarly spaced conserved cysteine residues. Except for EGF, this protein family also includes TGFα, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparinbinding, EGF-like growth factor (HBEGF), epigen, and the neuregulins (NRG)1- 6. The full-length 1133 amino acid (aa) rat EGF precursor protein is a type I transmembrane protein that contains nine EGF domains and nine LDLR class B repeats. Mature rat EGF shares 70% and 77% aa sequence identity with mature human and mouse EGF, respectively. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, are ligands for EGF family members. Depending on the context, EGF binds ErbB1 or ErbB2 to form homodimers or heterodimers of ErbB1/ ErbB2. Dimerization leads to autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules. EGF plays an important role in epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation.