More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 15.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat Fibronectin standard: 2 vials
2. One 96-well plate precoated with anti- Rat Fibronectin Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Fibronectin is an approximately 440kDa glycoprotein which plays a primary role in embryonic development, cell adhesion, growth, migration, differentiation, and wound healing. Fibronectin exists as a protein dimer, consisting of two nearly identical subunits. Each subunit is composed of three types of modules: type I, II, and III. There are two types of fibronectin in vertebrates: soluble plasma fibronectin and insoluble cellular fibronectin. Soluble plasma fibronectin produced in the liver by hepatocytes is a major protein component of blood plasma. Insoluble cellular fibronectin is secreted by various cells, primarily by fibroblasts, as a soluble protein dimer which is then assembled into an insoluble matrix in a complex cell-mediated process. The insoluble matrix formed by the cellular fibronectin is a major component of the extracellular matrix. Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and many integrin receptors.