More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 2.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat BMP-2 standard: 2 vials
2. One 96-well plate precoated with anti-Rat BMP-2 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Bone morphogenetic protein 2 (BMP-2), also known as bone morphogenetic protein 2A (BMP 2A), is a member of the BMP family of the TGFβ superfamily. BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region. Mature monomeric BMP-2 is an 18 kDa glycoprotein with seven conserved cysteines that form a cysteine knot structure. Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and BMP-2/BMP-7 heterodimers which are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo. Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. BMP-2 binds to Activin RI (BMPRIA, or BMPRIB) and BMP RII (Activin RIIB) to activate the downstream signaling pathways. BMP-2 plays an important role in inducing chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair.