Rat BDNF ELISA Kit

Rat BDNF ELISA Kit

FM-E100006

96T

31.2pg/mL-2000pg/mL

Less Than 2pg/mL

No detectable cross-reactivity with any other cytokine.

Store at 4 for frequent use, at -20 for infrequent use.

Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Four months at 4 and eight months at -20.

cell culture supernates, serum and plasma(heparin, EDTA, citrate).

 

 

1. Lyophilized recombinant rat BDNF standard: 10ng/tube-2.

2. One 96-well plate precoated with anti- rat BDNF antibody.

3. Sample diluent buffer: 30 ml

4. Biotinylated anti- rat BDNF antibody: 130µL, dilution 1:100.

5. Antibody diluent buffer: 12ml.

6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.

7. ABC diluent buffer: 12ml.

8. TMB color developing agent: 10ml.

9. TMB stop solution: 10ml.

 

 

Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.

 

 

Rat BDNF ELISA Kit-1X96 Well Plate Image