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Assay Range
3.12 - 100 pg/mL
Sensitivity
0.5 pg/mL
Assay Principle
Sandwich ELISA
Storage
Store at 2 - 8 °C
Sample Type
Serum, plasma, body fluids, tissus lysates
Size
96T
Sample Volume
50 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Rat IFN alpha standards (ready to use): 0.5ml/vial x 6.
2. One 96 well plate precoated with anti-Rat IFN alpha antibody.
3. Sample diluent buffer: 6 ml x 1
4. HRP conjugated detection antibody: 10 ml x 1.
5. Closure plate membrane: 2.
6. 20 x Wash buffer: 25 ml x 1.
7. TMB color developing agent A: 6 ml x 1.
8. TMB color developing agent B: 6 ml x 1.
9. TMB stop solution: 6 ml x 1.