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1. Rat HIF-1αstandard: | 20 ng/vial -2. |
2. One 96-well plate pre-coated with anti-Rat HIF-1αAb: | 1. |
3. Sample diluent buffer: | 12 mL - 1. |
4. Detection antibody: | 130 µL, dilution 1:100. |
5. Streptavidin-HRP: | 130 µL, dilution 1:100. |
6. Antibody diluent buffer: | 12 ml. |
7. Streptavidin-HRP diluent buffer: | 12 ml. |
8. TMB developing agent: | 10 ml. |
9. Stop solution: | 10 ml. |
10. 20 - Wash Solution: | 25 ml. |