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Rat Endothelin 1 ELISA Kit
BG-RAT10838
Rat Endothelin 1 (Edn1) ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction Competition
Data sheet
| Assay time | 4.5 hours |
| Assay Range | 3.9 - 125 pg/mL |
| Expiration | 6 months |
| Assay principle | The Rat ET-1 ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Rat ET-1 specific antibody has been pre-coated onto 96-well plate. Rat ET-1 present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Rat ET-1 detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Rat ET-1 captured on plate. |
More info
Rat Endothelin 1 (Edn1) ELISA Kit
Different lot may vary, please follow the instruction coming with the kit.