Rat MMP-9/ Gelatinase B ELISA Kit

The kit is used to quantify the rat matrix metallopeptidase 9 (MMP-9) in cell culture supernatant, serum, and other suitable sample solution. Does not show cross-reactivity with MMP-8, TIMP1 and Lipocalin-2. Less than 4% cross-reactivity with mouse and human MMP9.

Standard range             78.1 - 5000 pg/mL

Assay time                      4 h 40 min

Validity                            6 months

Store at                           2-8 °C

Background:

Aliases: Gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, CLG4B, GELB.

Matrix metallopeptidase 9 belongs to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix. They are synthesized as pro-enzymes and most are secreted before conversion to their active form. Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. MMP9, along with elastase, appears to be a regulatory factor in neutrophil migration across the basement membrane. It is also reported to play a role in angiogenesis, neovascularization and wound repair.

FOR RESEARCH USE ONLY