YARS2 Human

Data sheet

Formulation

YARS2 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl, 1mM DTT.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

YARS2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 482 amino acids (17-477 a.a.) and having a molecular mass of 53.7kDa.YARS2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary ch

Protein Background

Tyrosyl-tRNA synthetase (YARS2) is a mitochondrial protein which catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and afterward transferred to the acceptor end of tRNA(Tyr). YARS2

Expression host

Escherichia Coli.

Synonyms

Tyrosine--tRNA ligase mitochondrial, Tyrosyl-tRNA synthetase, TyrRS, YARS2, CGI-04, TYRRS, MLASA2, MT-TYRRS.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MTLNLSVLLP LGLRKAHSGA QGLLAAQKAR GLFKDFFPET GTKIELPELF DRGTASFPQT IYCGFDPTAD SLHVGHLLAL LGLFHLQRAG HNVIALVGGA TARLGDPSGR TKEREALETE RVRANARALR LGLEALAANH QQLFTDGRSW GSFTVLDNSA WYQKQHLVDF LAAVGGHFRM GTLLSRQSVQ LRLKSPEGMS LAEFFYQVLQ AY